Oncogenic factors facilitate a metabolic switch in order to transform a normal into cancerous cell

AWe aim to prove that ras is a direct regulator of de novo fatty acid synthesis pathways. Primary mouse embryonic fibroblasts (MEF) are immortalized by SV40 transduction and then infected with retrovirus encoding K- and H-ras proteins After selection, cells are tested for proliferation, apoptosis and changes in lipid and oxygen metabolism using techniques in routine in our group.


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