RESEARCH & TECHNOLOGY

  • The role of lactate in ischemic cerebral stroke

    We are studying the role of lactate in ischemic cerebral stroke (supported by the Biaggi and Juchum Foundations). We showed that administration of exogenous lactate improves the outcome after cerebral ischemia both in vitro and in vivo by reducing the extent of neural damage and neurological impairment. We are exploring the cellular and molecular mechanisms underlying this neuroprotection using molecular and pharmacological approaches as well as with Dynamic Nuclear Polarization of 13C-Lactate before magnetic resonance spectroscopy, in collaboration with Drs Jean-Noel Hyacinthe and Mor Mishkovsky, CIBM, EPFL and HESO, Geneva) (supported by FNS grant 310030_170155 / 1 / 1)

    TTC staining showing the lesion (white) and the healthy tissue (red) 24h after MCAO on a mouse treated with vehicle (left panel, control) or with L-lactate (right panel). Note the significant reduction in the size of the lesion after lactate treatment.

     

  • The role of caveolins after ischemic stroke

    It is well known that neurons are vulnerable to cerebral ischemia. We are taking a step back to study ischemia-induced changes at the level of the neurovascular unit. More specifically, we are investigating the role of caveolins after ischemic stroke and will correlate observations in mice after transient middle cerebral artery occlusion with changes occurring after traumatic brain injury, modelled in mice in collaboration with Dr. Jerome Badaut, CNRS INCIA Bordeaux; supported by FNS grant 31003A_163465 / 1)

    Endogenous Cav-1 and Cav-AP injection could be beneficial for outcomes after stroke and traumatic brain injury (TBI) by inhibiting JNK and eNOS in the brain endothelium.
  • Pericytes and stroke

    An intriguing new line of research is looking at the role of pericytes in stroke induced break-down of the blood-brain barrier.

    PDGFR beta-1 (a marker for pericytes, red) and GFAP (here labeling perivascular astrocytes, green) staining of an area in the lesioned brain 48h after MCAO.